Two elite wheat (Triticum aestivum L.) cultivars
Zhoumai 18 and Yumai 34 were used as the material for
mature embryo culture. The seeds were surface-sterilized
for 5 min in 75% (v/v) ethanol and rinsed three
times with sterile, deionized water, treated for 25 min
with 0.1% (v/v) HgCl2, and rinsed five times with deiondeionized
water. Seeds were left in sterile water for 19-20 h
to imbibe, and then the embryos were excised