Several studies were oriented towards quantisation (Table 1, J)
but the validation tests were not always carried out (Table 1, K).
Moreover, p-coumarate esters (Table 1, H) and minor cis isomers
(Table 1, I) co-elute with abundant ferulates and trans isomers in
RP-HPLC (Norton, 1995; Seitz, 1989; Yu et al., 2007) so that they
may affect the actual value of each integrated peak. Often the
peaks were not resolved to the baseline of the chromatogram
(Hakala et al., 2002); in such a case, total c-oryzanol was quantitated
by pooling all the peak area.
Several studies were oriented towards quantisation (Table 1, J)but the validation tests were not always carried out (Table 1, K).Moreover, p-coumarate esters (Table 1, H) and minor cis isomers(Table 1, I) co-elute with abundant ferulates and trans isomers inRP-HPLC (Norton, 1995; Seitz, 1989; Yu et al., 2007) so that theymay affect the actual value of each integrated peak. Often thepeaks were not resolved to the baseline of the chromatogram(Hakala et al., 2002); in such a case, total c-oryzanol was quantitatedby pooling all the peak area.
การแปล กรุณารอสักครู่..
