Preparation of plant extract
In vivo leaves of M. azedarach collected from source plant were washed for 2-3 times with tap water and finally with distilled water, followed by ethanol wash and then allowed to dry at 50o
Microbiological screening C for overnight and finally milled to a coarse powder. 100 gm of powdered material was soxhlet extracted with different solvents like, Ethanol, methanol, petroleum ether and aqueous (12 hour each). All the extracts were evaporated in vacuum under reduced pressure. All extracts were stored in sterile glass bottles at room temperature until screened.