A hyperuricemia mouse model was adopted from the literature (28,29) Allantoxanamide, a potent uricase inhibitor , was use to induce hyperuricemia in mice [29]. Briefiy,adult C57BL/6 mice [15-25 g,6-8 week old ,6 per group:Jackson Labs] receive DHNB at a concentration of 100 mg/kg 1.0% polyethylene glycol 400 (PEG400 in a volume of 0.1 ml/10g mouse body weight) via oral gavage.