The liquid state 13C NMR spectrum of the isolated BG was
recorded in D2O on a Bruker DPX-400 instrument(Billerica, Bruker,
MA, USA) operating at 400 MHz andthe chemical shifts inppmwere
normalized as downfield values from that of internal standard, TSP
(sodium 2,2,3,3-tetradeuterio-3-(trimethylsilyl)-propionate).