Total soluble phenolic content of A. paniculata and T. cordifolia was estimated by Folin-Ciocalteu’s
reagent method14 using gallic acid as a standard phenolic compound. Each solvent extracted solution (0.3
mL in triplicate) was mixed with 1.5 mL of 10% freshly prepared Folin-Ciocalteu’s reagent and after 3 min,
1.2 mL of 7.5% (W/V) sodium carbonate was added and mixed thoroughly. The tubes were placed in boiling
water for one minute, cooled and the absorbance was measured at 650 nm in a spectrophotometer against a
reagent blank. The concentrations of the total phenolic compounds in the extracts were obtained by
extrapolating the absorbance of gallic acid on standard gallic acid graph. The experiment was repeated thrice
and concentration of total phenols was expressed as mg/g of dry extract.