E. coli and S. pyogenes determined viable bacteria counts were
used for DNA extraction. Bacterial pellets of 5.0 107, 5.0 106,
5.0 105, 5.0 104, and 5.0 103 CFU were resuspended in 180 mL
of the enzyme solution and bacterial DNA was eluted in 100 mL
Buffer AE. These purified DNA samples were included as standards
for each PCR run