16s rRNA amplification reaction was performed using 16S
primers 27F and 1492R in a 0.2 mL optical-grade PCR
tube (Tarsons, India). 50 ng of DNA extract was added to a
final volume of 50 lL of PCR reaction mixture containing
1.5 mM MgCl2, 19 Reaction buffer (without MgCl2)
(Fermentas), 200 lM of each dNTPs (Fermentas), 100 pM
of each primer and 1.5 U Taq DNA polymerase (Fermentas).