Methanogens diversity was investigated by using PCR–
DGGE technique and gel image and are shown in Fig. 2. It was
found that, methanogens diversity are not different among
treatments and time at 0–4 h post feeding. However, 13
separate bands in the gel were found. Under this study,
methanogen diversity was not changed when supplemented
with coconut oil and garlic powder. Although, the diversity
was not changed but the number of population was changed.
The predominant species of methanogens in swamp buffalo
have not yet been identified while in sheep and cows. The
methanogens were Methanobacteriales, Methanosarcinales,
Methanobrevibacter and Methanomicrobiales, respectively