Effect of contact time on cell disruption during mixing of polymers and enzymes with microalgal cells. The polymer used in this study was pNcAA-5 with p
olymer loading of 0.2 g/
g BDW. Enzyme loading = 6% (g enzyme/g total working weight, equivalent to 0.1 g EP/g BDW) which was added to cell suspension 1 h after polymer addition. T
he algae concentration =
10 mg/mL, pH = 4.8, mixing speed = 120 rpm, and temperature = 30 °C.