Extraction from de-hulled hemp seeds was experimentally determined using supercritical fluid extraction equipment (ISASCCO-S-050-500, Ilshin Co. Ltd., Republic of Korea). About 100 g of de-hulled hemp seeds were loaded into a stainless steel extraction vessel. CO2 was pressurized with a high-pressure pump and then charged into the extraction column to desired pressure. Back pressure regulators are used to set the system pressure (in extractor and separator). The SF was conducted at the temperature of 40 องศาเซลเซียส, and pressure of 400 bar. The CO2 flow rate was maintained at 30 mL/min and the extract was collected in a glass tube. The extraction process was performed for 60 min and the yield of SF extract was expressed as percent of the dry weight of seeds.