In the present study, we have demonstrated that, using the response surface methodology approach for optimization, BC can be produced in shake flask culture at a rate of 1.51 g/l with maple syrup as carbon source, which is comparable to that using pure fruc- tose as the carbon source (1.60 g/l). Therefore maple syrup can be used effectively as an alternative to fructose in commercial scale BC production. In major maple syrup producing countries such Canada and the United States, the use of maple sap collected from the trees directly for fermentation is even more attractive. Canada alone produces more than 80% of the world’s maple syrup, about 7 million gallons in 2005 and there is room for further expansion of its production (Keough, 2009). This represents an effective use of a plentiful and renewable resource in the form of either maple syrup or maple sap for the production of a nanobiomaterial suitable for a broad range of biomedical and other applications.