2. Materials and Methods
2.1 Isolation of Aspergillus niger
Aspergillus niger strains were isolated from the following samples: Soil, Window
glass, corridor floor and Microbiology Lab. To isolate Aspergillus niger strain from the
collected samples, Potato Dextrose Agar (PDA) was used. Composition of Potato
Dextrose Agar (PDA) is Potato, 100g; Dextrose, 10g; Agar, 10g and distilled water,
500ml. pH: 5.6 ± 0.2 at 250C.