1. Although the choice of diluents might differ among practitioners,
the use of either preservative-free normal saline
for intravenous injection or 4% milk is preferred to dilute
the stool sample.
2. For best results, a conventional household blender (dedicated
to this purpose) should be used. The stool should
be homogenized, adding more diluent as necessary, until
it reaches a liquid slurry consistency.
3. The stool should be filtered to remove as much particulate
matter as possible. This can be accomplished by using a number
of methods (eg, gauze pads, urine stone strainers).
4. The finished stool slurry should be used immediately.
5. The ideal volume for instillation has not been established.
However, smaller volumes (eg, 25–50 mL) should
be used for delivery from above; larger volumes (eg, 250 –
500 mL) should be used for delivery from below.