Obtaining of FFAs from UVO and microalgal biomass FFAs fromUVO were
obtained by saponification of the oil. The reaction mixture contained 350 mL of oil
and 700 mL of a hydroalcoholic solution of NaOH, which was prepared dissolving
120 g of NaOH in 400 mL of water and 400 mL of ethanol (96% v/v). The saponification
was carried out shaking this mixture at 200 rpm, at 60C for 30 min. The
mixture was then cooled at room temperature, 140 mL of water were added and the
pH was adjusted to values between 1 and 2 using 37% HCl. FFAs were extracted with
300 mL of hexane, shaking at 200 rpm for 30 min. Finally the hydroalcoholic and
hexane phases were separated by decantation and the hexanic phase was washed
twice with water.