The precipitate
at pH 4.8 was extracted with deionized water (1:20, w/w, while the
pH was with 1 M NaOH), and the resulting suspension was centrifuged
at about 10,000g for 30 min, dialyzed against distilled
water, and then freeze-dried to recover a second b-conglycinin–d
extran conjugate in macromolecular crowding environment
(MC65).
The precipitateat pH 4.8 was extracted with deionized water (1:20, w/w, while thepH was with 1 M NaOH), and the resulting suspension was centrifugedat about 10,000g for 30 min, dialyzed against distilledwater, and then freeze-dried to recover a second b-conglycinin–dextran conjugate in macromolecular crowding environment(MC65).
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