Only the traditionally consumed portions of the fruit were used in the extraction procedure.
For example, kiwi were peeled while the apples remained unpeeled but cored. Vitamin C was
extracted, from the fruit, into solution using pH 2.0 phosphate buffer, which was prepared daily with
0.1 M KCl, 0.1 mM EDTA, 0.1 M sodium phosphate monobasic and adjusted to the proper pH with
phosphoric acid as outlined by Ogunlesi et al. [14]. Extraction was performed by combining 50 g of
fruit with 50 mL of buffer solution and blending for 1 minute. An additional 50 mL of buffer was used
Int. J. Electrochem. Sci., Vol. 5, 2010 1467
to wash the unblended residue along the inside of the blender, producing 100 mL of solution. This
solution was further blended until a uniformly smooth consistency was achieved. The blended mixture
was then vacuum filtered to remove pulp, resulting in a transparent solution. The filtrate was degassed
with nitrogen for 5 minutes and immediately tested.
Only the traditionally consumed portions of the fruit were used in the extraction procedure.For example, kiwi were peeled while the apples remained unpeeled but cored. Vitamin C wasextracted, from the fruit, into solution using pH 2.0 phosphate buffer, which was prepared daily with0.1 M KCl, 0.1 mM EDTA, 0.1 M sodium phosphate monobasic and adjusted to the proper pH withphosphoric acid as outlined by Ogunlesi et al. [14]. Extraction was performed by combining 50 g offruit with 50 mL of buffer solution and blending for 1 minute. An additional 50 mL of buffer was used Int. J. Electrochem. Sci., Vol. 5, 2010 1467to wash the unblended residue along the inside of the blender, producing 100 mL of solution. Thissolution was further blended until a uniformly smooth consistency was achieved. The blended mixturewas then vacuum filtered to remove pulp, resulting in a transparent solution. The filtrate was degassedwith nitrogen for 5 minutes and immediately tested.
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