This inconvenience was already observed for I. tinctoria and, at a lower extent, in I. indigotica ( John and Angelini, 2009); consequently, the achieved seed yields were lower than the potential productive capability. Furthermore, with the exception of II1, that showed a 100% of plants turning flowering, part of the plants of the other accessions remained in the rosette vegetative stage and did not differentiated a flowering stalk determining a consequent seed yield reduction. This behavior could be due to some genetic instability of the tested genotypes and the possibility of cross-pollination with other Isatis species (namely I. tinctoria) as already reported by John and Angelini (2009). In fact, it was shown (John and Angelini, 2009) that Isatis spp. needs cold temperatures for their vernalisation. On the other hand,I. indigotica is more sensitive to vernalizing triggers than I. tinctoria ( John and Angelini, 2009), presumably attributable to its adaptation to a warmer climate compared with that I. tinctoria is adapted to. Low temperatures (