The asparaginase solution thus obtained is then puri?ed in a
conventional manner. For example, salting out the enzyme
with a salt such as ammonium sulfate; precipitating the enzyme with an organic' solvent such as alcohol or acetone;removing nucleic acid using protamine, streptomycin, magnesium ion or the like; column chromatography using ion exchange material such as ion exchange resin or ion exchange cellulose; gel ?ltration using “Sephadex" (dextran) or “Biogel" (polyacrylamide); electrophoresis, etc. Other equivalent methods will suggest themselves to those skilled in the art.