Parent hens were maintained outdoors
under free-range conditions or in pavilions, respectively. In both conditions,
according to the information provided by the company in eggs
box, laying hens were provided with animal origin food, also designated
as commercial feed, meaning that commercial feed was made of b60%
cereals, according to the Portuguese legislation (Regulamento (CE) no
589/2008, de 23 de Junho, artigo 150, alínea a). The amount of food provided
to the hens is controlled by the company that raises the hens. All
eggs were laid in late August.
Four eggs from all different conditions
were randomly selected. Egg yolks were separated manually from the
albumen.