Kinetic traces for the deacetylation of DA-ZP1-TPP. The half-life for
deacetylation of a 2.75 μM solution of DA-ZP1-TPP at 37 °C in buffer (50 mM
PIPES, 100 mM KCl; pH 7), was measured in the presence of 125 μM ZnCl2
(black circles), 250 μM EDTA (triangle), or 1.25 U of porcine liver esterase
(white circles). The change in absorbance at 520 nm (EDTA and esterase) or
510 nm (ZnCl2) was used to monitor the reaction.