Mycotoxins are contaminants of animal feed that can impair fertility and cause abnormal fetal development in farm animals. The aim of the present
study was to investigate the influence of Fusarium-toxin contaminated feed on cumulus morphology and maturation of pig oocytes. Naturally with
the Fusarium-toxins deoxynivalenol (DON) and zearalenone (ZON) contaminated wheat was included in feed for gilts at increasing proportions
which resulted in increasing dietary concentrations of both toxins (in mg toxin/kg feed: Group 1 (control), 0.21 and 0.004; Group 2, 3.07 and 0.088;
Group 3, 6.1 and 0.235; Group 4, 9.57 and 0.358, for DON and ZON, respectively). Oocytes were recovered from gilt ovaries by follicle aspiration
after ovario-hysterectomy. Granulosa cells were analyzed for the expression of the P450SCC and 3-HSD mRNA by RT-PCR and additionally for
P450SCC protein by Western blotting. Neither the expression of the P450SCC nor of the 3-HSD mRNA or the abundance of the P450SCC protein
was significantly influenced by the mycotoxin application. The distribution of different cumulus cell morphologies was not influenced by group.
At the time of recovery, oocytes with compact cumuli in Groups 3 and 4 showed a reduced proportion having immature chromatin in comparison
to that for Groups 1 and 2. The proportion of oocytes having degenerated meiotic chromatin was significantly higher in Group 4 than in the other
groups. The proportion of oocytes reaching metaphase II in culture was significantly lower in Groups 3 and 4 than in Group 1, and tended to be
lower in Group 2 than in Group 1. We conclude that oocyte quality is significantly reduced by feeding of Fusarium-toxins to gilts.
© 2005 Elsevier Inc. All rights reserved.
Mycotoxins are contaminants of animal feed that can impair fertility and cause abnormal fetal development in farm animals. The aim of the presentstudy was to investigate the influence of Fusarium-toxin contaminated feed on cumulus morphology and maturation of pig oocytes. Naturally withthe Fusarium-toxins deoxynivalenol (DON) and zearalenone (ZON) contaminated wheat was included in feed for gilts at increasing proportionswhich resulted in increasing dietary concentrations of both toxins (in mg toxin/kg feed: Group 1 (control), 0.21 and 0.004; Group 2, 3.07 and 0.088;Group 3, 6.1 and 0.235; Group 4, 9.57 and 0.358, for DON and ZON, respectively). Oocytes were recovered from gilt ovaries by follicle aspirationafter ovario-hysterectomy. Granulosa cells were analyzed for the expression of the P450SCC and 3-HSD mRNA by RT-PCR and additionally forP450SCC protein by Western blotting. Neither the expression of the P450SCC nor of the 3-HSD mRNA or the abundance of the P450SCC proteinwas significantly influenced by the mycotoxin application. The distribution of different cumulus cell morphologies was not influenced by group.At the time of recovery, oocytes with compact cumuli in Groups 3 and 4 showed a reduced proportion having immature chromatin in comparisonto that for Groups 1 and 2. The proportion of oocytes having degenerated meiotic chromatin was significantly higher in Group 4 than in the othergroups. The proportion of oocytes reaching metaphase II in culture was significantly lower in Groups 3 and 4 than in Group 1, and tended to belower in Group 2 than in Group 1. We conclude that oocyte quality is significantly reduced by feeding of Fusarium-toxins to gilts.© 2005 Elsevier Inc. All rights reserved.
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