All yeast transformants obtained were initially screened in YPD
media for improved heterologous protein production by determining
enzyme activity of the yeast supernatant in liquid assays with
lichenin, carboxymethylcellulose (CMC) and p-nitrophenyl-b-Dglucopyranoside
(pNPG) as substrates for Y294[cel6A], Y294[cel7B]
and Y294[cel3A], based strains respectively, using modified protocols
as described previously [6,33,34].