The liquid samples from hydrolysis and SSF were analyzed byhigh performance liquid chromatography (HPLC) equipped withUV/vis and RI detectors (Jasco International Co., Tokyo, Japan).Ethanol and glycerol concentrations in the SSF samples weredetected on an Aminex HPX-87H column (Bio-Rad, Richmond, CA,USA) at 60◦C with 0.6 ml/min eluent of 5 mM sulfuric acid. Theconcentration of sugars in the enzymatic hydrolysates and carbo-hydrate analysis were analyzed on an Aminex HPX-87P column(Bio-Rad, Richmond, CA, USA) at 85◦C using deionized water as amobile phase with flow rate of 0.6 ml/min.