Materials And Methods
2.1. Collection of leaves and preparation of leachate of Albizia saman Dicot leaves of vigorously growing individuals of Albizia saman were collected from certain areas of Khanyan under Hooghly District (West Bengal) during January to April 2012, 2013. Leachates were prepared by soaking 1 gm of dried leaves in 100 ml of sterile distilled water for 72 hours. Filtrate was taken in a volumetric flask. This is 1% leachate. In this way, 2% and 4% leachate was made.