In our laboratory, we found that crude flower extract
of Caesalpinia pulcherrima possesses an impressive anti-
oxidant activity using DPPH radical scavenging assay
with an EC50 of 0.05 mg/mL. We also formulated an anti-
wrinkle cream containing crude extract of Caesalpinia
pulcherrima flower in oil-in-water (O/W) emulsion. It was
found that antioxidant activity of the cream was
adequately stable under accelerated condition (%AA =
71.8 ± 1.8) (unpublished research report of a pharmacy
undergraduate project, under supervision of two N.
Kamkaen and W. Samee). In order to assure safety of
the anti-wrinkle cream, we conducted skin irritation tests
on rabbit and human in this present study.