Briefly, diatoms in the late exponential phase were filtered and resuspended in 0.2-mm water containing f / 2 levels of N, P, Si, and vitamins, and f / 2 levels of trace metals without additions of EDTA, Cu, and Zn. Radioisotope additions were 37 kBq l21 for
109Cd, 55.6 kBq l21 for 51Cr, and 37 kBq l21 for 65Zn. After 4 days of growth, the diatom cells were collected by 3-mm polycarbonate membranes before resuspension in
30 ml 0.2 mm of filtered seawater.
Briefly, diatoms in the late exponential phase were filtered and resuspended in 0.2-mm water containing f / 2 levels of N, P, Si, and vitamins, and f / 2 levels of trace metals without additions of EDTA, Cu, and Zn. Radioisotope additions were 37 kBq l21 for109Cd, 55.6 kBq l21 for 51Cr, and 37 kBq l21 for 65Zn. After 4 days of growth, the diatom cells were collected by 3-mm polycarbonate membranes before resuspension in30 ml 0.2 mm of filtered seawater.
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