Wee et al. (1992) concluded that, for P. coronaries and P. ridleyi, rooting and acclimatization abilities are species dependent, so rooting treatment was not applied in their experiments. We therefore omitted the procedure for rooting of regenerates in this study and carried out testing to determine its necessity. We observed spontaneous rooting displayed by the in vitro grown non-rooted propagules of P. bifurcatum, which again proved that this fern species is relatively high in competency. Plants received acclimatization achieved acceptable rooting percentages and cloud survive after being directly transplanted into the potting media in a shaded greenhouse, which allowed for additional savings of equipment, labor and time.