The ALA- and OF-induced changes in expression of genes encoding the detoxifying proteins in leaves of rice plants. The plants were subjected to the same reatments as in Fig. 1.
Treatment notations are the same as in Fig. 1. Aqua, aquaporin; NaþeHþ, Naþ/Hþ exchangers; SereThr, serine/threonine kinase receptor; Chap, chaperone; Iron-Asc, iron- and ascorbate family oxidoreductase. Total RNAs were purified from plants and reverse transcribed.
The resultant cDNAs were used as templates for qRT-PCR using Actin as an internal control. The control was used for
normalization, with the expression level of the sample set to 1. Error bars represent SE, and representative data from three independent experiments are presented