ฉันรักแปลSegments taken from young leaves of an
orchid (Oncidium Gower Ramsey) produced clusters of
somatic embryos directly from epidermal and mesophyll
cells of leaf tips and wound surfaces without an
intervening callus within 1 month when cultured on a
GelriteTM-gelled 1/2-MS basal medium supplemented
with a low dosage (0.3–1 mg/l) of thidiazuron. Subculturing
of these embryo clusters produced more
embryos and subsequent plantlet formation on the
same medium. The high-frequency embryogenesis of
these leaf cells in this orchid is strong evidence of their
totipotency, and further modification of the protocol
for plant formation could be useful for the mass propagation
and transformation of selected elite lines.