and performed experiments in order to determine their roles in transcriptional activation of the bovine Nanog expression. Results from promoter assay, electrophoretic mobility shift assay (EMSA) and chromatin-immunoprecipitation (ChIP) assay revealed that Sp1 transcription factor regulates the bovine Nanog proximal promoter activity through direct binding to a Sp1-binding site in the bovine Nanog proximal promoter, whereas NF-κB, which plays an important role in transcriptional activation of the Nanog expression in both human and murine ESC, is dispensable in the bovine Nanog promoter activity. and performed experiments in order to determine their roles in transcriptional activation of the bovine Nanog expression. Results from promoter assay, electrophoretic mobility shift assay (EMSA) and chromatin-immunoprecipitation (ChIP) assay revealed that Sp1 transcription factor regulates the bovine Nanog proximal promoter activity through direct binding to a Sp1-binding site in the bovine Nanog proximal promoter, whereas NF-κB, which plays an important role in transcriptional activation of the Nanog expression in both human and murine ESC, is dispensable in the bovine Nanog promoter activity.