2.2. Sample preparation from Yarrowia lipolytica Po1g biomass Biomass sample was prepared based on the established procedures of our previous work [32]. Cultivation of Y. lipolytica Po1g
cells was performed in 500 mL conical flasks each containing 250 mL culture media in an orbital shaker incubator model LM-570 at 26 °C and 160 rpm. The biomass was collected, separated from the aqueous phase by centrifugation and then subjected to freeze drying. The freeze-dried biomass sample was then collected and stored at 4 °C before use.