These sequences were then filtered, and 17,801 optimized sequences (90%) with average lengths of 538 bp were retained (6300 for Ft1y, 6449 for Ft2y, and 5052 for Ft3y). This indicated that the design of the 454 sequencing was reasonable and sufficient, and it could thus be used for analyzing the diversity of fungi in soybean rhizosphere soil. The optimized sequences were divided into 757 OTUs of