In 1968, Delahunty and Cherry5 reported the formation of
laryngeal granuloma in the canine vocal cord: they induced
the granuloma by placing a cotton ball moistened with gastric
juice (obtained by aspiration through a gastric tube) on
the canine vocal cord for 20 minutes daily over 39 days.
This was the first report on the association between gastric
acid reflux and laryngeal granuloma in an animal model.
Here, we report the development of laryngeal granuloma
due to chronic reflux of endogenous gastric acid and
mechanical injury of the vocal cord mucosa in rats. In this
experimental model, the animals did not undergo surgery
along the esophago-gastric junction; consequently, their
defense mechanisms against regurgitation were preserved