This study was carried out to assess knowledge associated with traditional use of mosquito repellent plants in the Central Zone of Tigray, Northern Ethiopia. The present study demonstrates that the local inhabitants have knowledge concerning what they perceive as mosquito repellent plants. This is one of the major malaria endemic areas in Kolla Temben and the community was frequently exposed to malaria. Moreover, use of repellent plants was high among the local communities, where access to other alternative control options was less. The level of knowledge of the community is similar to a study done in other parts of the country, in which 97.2% of the community had knowledge and usage of mosquito repellent plants (Karunamoorthi et al., 2009). On the other hand, the majority (82.1%) of the local community believes plant repellents repel mosquito, while 10.9% believes the repellents kill and repel, 2.2% believes repellents kill and 2.2% believes repellents avoid malaria transmission. However, currently the practice of usage is very poor. This is probably due to access to health center, free distribution of impregnated bed nets and problem of accessibility of most of the mosquito repellent plants in the study area. The extracts from plant materials can be useful as