Antimicrobial activity testing Antimicrobial activities of the herb extracts
against S. agalactiae were examined using a swab paper disc method with some
modifications. Forty microliters of each herb extract were spotted on a sterile paper disc
(Schleicher and Schuell BioScience, Keene, NH, USA), placed onto a BHI agar plate,
spread with a swab of overnight culture containing colony forming units (CFU) of S.
agalactiae (approximately 108/mL)