Muller Hinton agar (Himedia, Mumbai) was prepared by dissolving 15.2 g in 400 mL of distilled water. Then the medium was sterilized by autoclaving at 121 °C and at 15 lb for 15 min. After sterilization, the medium and sterile Petri plates were transferred into laminar air flow chamber. Approximately 15-20 mL of the sterile medium was poured
in each sterile Petri plates. The medium was allowed to solidify in laminar flow and inoculated with the overnight bacterial cultures. The sterile Whatman fitter paper No.1 disc was prepared and discs were soaked in the plant extracts and then placed over the inoculated plates. After 24 h of incubation at 37 °C, the zone of inhibition around the disc was measured[7].