Nile tilapia was collected from a local
hatchery (Rupali Fish and Prawn Hatchery
Limited, Rawzan, Chittagong). The mean total
length (±SD) and body weight (±SD) of
experimental fishes were 5.32±0.050 cm and
4.24±0.106 g, respectively. Fishes were
acclimated for two weeks in plastic tank (50
litres of volume) and fed at 3-4% of their body
weight with control diet containing 35% crude
protein and 6.98% lipid. At the end of
acclimated period, fish were counted and
stocked at density of 30 fish per tank. Three
replicate tanks were used for each diet and
fishes were fed three times (07:00, 12:00 and
17:00 h) at a rate of 3-4% of their body weight
per day.