According to the results of initial shaken cultures, the C. tropicalis,
P. stipitis and C. guilliermondii strains were chosen for fedbatch
cultivations in a benchtop fermentor. The mean of the 3
cultivation results expressed as the ‘‘net’’ (with the subtracted
amount of inoculum) biomass concentration in the fermentor
[g DM] is shown in the Figure below (see Fig. 3).
During 10 h of cultivation, the ‘‘net biomass’’ (biomass without
seed yeast) content increased from 0 g DM (24 g DM of the inoculum
was subtracted) to 45.4(±4.43) g DM for C. tropicalis,
54.7(±6.35) g DM for P. stipitis, and 43.6(±5.87) g DM for C. guilliermondii
in the fermentor.
The crude protein content in the dry matter of C. tropicalis biomass
obtained after 10 h of fed-batch cultivation was 47.8(±2.34)%.