‘Bandera’, where we repeatedly proved a gametophytic
(initially haploid) origin for all the regenerants obtained
(Corral-Martı´nez and Seguı´-Simarro 2012). Genetic variation,
from gross ploidy differences to subtle genetic
changes, is a well-known phenomenon inherent to all
in vitro culture processes (reviewed in Dunwell 2010).
However, the consistency of the SSR analyses reported
hereby and previously published make us think that in this
particular case, genetic variation should not have a great
impact. In addition, *70 % of DHs is a percentage better
than previous results from microspore cultures in this and
other eggplant cultivars, which revolved around 60 %
(Miyoshi 1996; Corral-Martı´nez and Seguı´-Simarro 2012).
This percentage is much better than those obtained through
anther culture (46.4 % of DHs in Salas et al. 2011, 25.6 %
in Rotino 1996, 23.5 % in Tuberosa et al. 1987 and 15.4 %
in Dumas de Vaulx and Chambonnet 1982). Thus, it appeared
that the prolonged in vitro culture time did not affect
to the rate of occurrence of higher ploidies, while it
seemed beneficial in terms of reducing the rate of haploids.