Application of hydrolytic and other enzymes for improving fermentation performance and oil recoveryin corn dry-grind process was optimized. Non-starch polysaccharide enzymes (BluZy-P XL; predomi-nantly xylanase activity) were added at stages prior to fermentation at optimum conditions of 50◦C andpH 5.2 and compared with conventional fermentation (30◦C, pH 4.0). Enzyme applications resulted infaster ethanol production rates with a slight increase in yield compared to control. The thin stillage yieldincreased by 0.7–5% w/w wet basis with corresponding increase in solids content with enzyme treat-ment after liquefaction. The oil partitioned in thin stillage was at 67.7% dry basis after treatment withhydrolytic enzymes during fermentation. Further addition of protease and phytase during simultaneoussaccharification and fermentation increased thin stillage oil partitioning to 77.8%. It also influenced otherfermentation parameters, e.g., ethanol production rate increased to 1.16 g/g dry corn per hour, and thinstillage wet solids increased by 2% w/w. This study indicated that treatments with non-starch hydrolyticenzymes have potential to improve the performance of corn dry-grind process including oil partitioninginto thin stillage. The novelty of this research is the addition of protease and phytase enzymes dur-ing simultaneous saccharification and fermentation of corn dry-grind process, which further improvedethanol yields and oil partitioning into thin stillage.