Culture supernatant of B. atrophaeus OSY-7LA showed antimicrobial activities against all Gram-positive bacteria tested; these are L. monocytogenes, L. innocua, B. cereus, methicillin-resistant Staphylococcus aureus and Enterococcus faecalis ( Table 1). No activity was observed against the tested Gram-negative bacteria, E. coli, Yesinia enterocolitica and Salmonella Typhimurium. A fungus, R. arrhizus, was also tested and was found susceptible to OSY-7LA culture supernatant.