Brain Heart Infusion Agar (BHI Agar) is an enriched non-selective medium for the isolation and cultivation of most anaerobic bacteria and other fastidious microorganisms. The basic nutritive properties are brain heart infusion from solids as well as meat peptones,with the addition of yeast extract. This medium is supplemented with hemin and vitamin K1 as growth factors for most anaerobic bacteria. This medium is prepared, dispensed, stored and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.