Polymerase Chain Reaction amplification
A pair of universal primer, cytrochrome b1 and cytrochrome b2, which was published by Kocher et al. (1989) was used to amplify the cyt b gene. Primers sequences were as follows: forward (cyt b1), and reverse (cyt b2), The primers were purchased from reserch Biolabs. Reactants for amplification were combined in 0.5 ml polypropylene tubes to give a final volume of 50. Each reaction mixture contained 200 nucleotilde (dNTP) mix