The Spread isolated bacteria were six late varieties onto LB agar be incubated for 24 hours
then use a paper disc containing the extract of the herb , the fifth of which is a separate sheet of one kind placed on LB agar. 1 Petri dish in which to place the disc 1 plate
then place the paper disc Petri dish is incubated at room temperature. 37 ° C for 24 hours (the test is repeated with each strain of bacteria three times).