This work relates the structure and properties of soy protein-based films and provides an alternative and novel material to produce renewable packaging with less environmental impact in climate change and fossil fuels categories than the conventional films available in the market. Biodegradable films were produced with soy protein obtained as a by-product from soy oil industry. The effect of plasticizer content was analyzed to optimize formulations and processing conditions were optimized to improve functional properties. Furthermore, the changes observed in the final properties of the films were related to the structural changes in raw materials.