Airborne particulate matter derived from feed yards facilitated dispersal of several veterinary antibiotics, and microbial communities containing ARG. Concentrations of several antibiotics in airborne particulate matter immediately downwind of feedyards ranged from 0.5-4.6 μg/g of particulate matter. Microbial communities of particulate matter collected downwind of feedyards were enriched with ruminant-associated taxa, and were distinct when compared to upwind PM assemblages. Furthermore, abundance of genes encoding resistance to tetracycline antibiotics was significantly greater in particulate matter collected downwind of feed yards as compared to upwind.