The
suspension was serially diluted 10-fold, and
100 µl per dilution were spread onto nutrient
agar plates. Three infiltrated leaves were
used as replications from each time interval.
The colonies on each plate were counted
after incubation at 28°C for 72 h. The experiment
was conducted twice.
Carbon utilization and fatty acid
analysis. Pure cultures of isolates 6269
and 6369 were grown and tested for utilization
of the 95 carbon sources available
on GN Microplate (Biolog Inc., Hayward,
CA) as previously described (3). The
carbon utilization patterns were read with
a microplate reader and analyzed by a
cluster analysis program provided by
Biolog.