2.3. DNA extraction and genomic sequencing1
Digestate samples withdrawn from the pilot apparatus were
processed for DNA extraction using the TruSeq™ preparation kit
v2 (illumina). Successively, DNA was: fragmented and tagged using
the Nextera DNA sample preparation kit (illumina); quality controlled
and PCR amplified; hybridized and amplified for cluster
generation using cBot (illumina) and finally processed in an illumina
MiSeq HTS (Li et al., 2014; Yang et al., 2014).