HyPhy (Kosakovsky Pond et al. 2005) were employed to
provide additional support to the aforementioned analyses
and to detect sites evolving under the influence of positive
and negative selection. Mixed Effects Model Evolution
(MEME) (Kosakovsky Pond et al. 2011) was also used to
detect episodic diversifying selection. Since, the three
domains of the coleoid pacifastin gene are expressed as